Pseudo-symmetry detection using self-rotation function?

Ian Berke berkei at rockefeller.edu
Mon Dec 22 10:40:39 EST 2003


Hi,

I suspect that a protein I am studying is a B-propeller but I am
unsure how many repeats it contains (6 vs 7).  I have a native data
set to 2.9A and would like to know if it is possible to detect the
symmetry of the propeller by a self-rotation function search for peaks
@ 60 and 51.4 deg.  If possible, how sensitive to the resolution would
this be?  Are there any parameters I should be especially careful with
(integration radius, radius of removed patterson sphere, etc.)?

Thanks,
Ian Berke





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