[Protein-crystallography] help! membrane protein solubilization

aumium qasun at yahoo.com
Tue Jun 7 17:45:55 EST 2005


Hi, friends.

I have a membrane protein which consists of 12 TMs. I expressed it in
E. coli and isolated the membrane fraction. Then I tried to extract it
from the membrane with different detergent, but just failed even with
1%SDS.

Could anybody give me some clue? Thanks.




More information about the Xtal-log mailing list