[Protein-crystallography] Re: help! membrane protein solubilization

Marc Böhm maboehm01 at arcor.de
Thu Jun 9 16:42:50 EST 2005

aumium wrote:
> Hi, friends.
> I have a membrane protein which consists of 12 TMs. I expressed it in
> E. coli and isolated the membrane fraction. Then I tried to extract it
> from the membrane with different detergent, but just failed even with
> 1%SDS.
> Could anybody give me some clue? Thanks.


Did you tried also additional parameters like pH, salts, Temperature?

By the way, are you sure that your protein is properly integrated into 
the membrane. I had a target which remained in inclusion bodies inside 
the cell... even the strongest detergent would fail if the protein isn't 
part of the membrane fraction.



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