[Protein-crystallography] ATP affinity chromatography

Ruben Martinez-Buey rmartinez at cib.csic.es
Tue Jun 20 07:06:32 EST 2006

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 Dear all,
Has anybody experience with ATP-sepharose /ATP-agarose chromatography?
Is there any recommended protocol? How many times can I use the column? Is
the ATP in the column going to be hydrolized?
Thank you very much in advance for your help.
With my best regards,
 Ruben
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