[Protein-crystallography] Re: Lipase substrate analogue

Dr Engelbert Buxbaum engelbert_buxbaum at hotmail.com
Fri Sep 22 08:53:43 EST 2006


Clement Angkawidjaja wrote:

> Hi, I am working on a project for the crystallization of a bacterial lipase. 
> I got some good crystals on the native enzyme, and I also want to 
> crystallize the enzyme in complex with a substrate analogue (inhibitor). I 
> used the commercially-available paraoxon (also called E600), but no 
> inhibition occured. 

Have you checked the enzymology database BRENDA? You'll find fairly
complete information on activators and inhibitors of enzymes there,
amongst other things.



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