[Protein-crystallography] Re: I want to crystallize one protein with signal peptide on N-terminal, Is it possible?

Clement Angkawidjaja via xtal-log%40net.bio.net (by clement from bio.mls.eng.osaka-u.ac.jp)
Thu Nov 13 21:44:57 EST 2008


Hi Mitra,

The answer, like most answers about protein crystallization, is "It 
depends."

The thing is, most proteins with N-terminal signal sequence are not 
(completely) folded when the signal is intact. But since you mentioned that 
yours is active with the signal, it MAY crystallize. However, I would 
suggest that you perform crystallization screening using both the intact and 
signal sequence-removed constructs. This way you can save a lot of time if 
one of them does not crystallize well.

Regards,
Clement Angkawidjaja, Ph.D
Assistant professor
Laboratory of Molecular Biotechnology
Division of Advance Science and Biotechnology
GSE Common East 8F
2-1 Yamadaoka, Suita-shi, Osaka, 565-0871, Japan
Tel/fax: +81-6-6879-4580

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Sent: Friday, November 14, 2008 2:03 AM
Subject: Xtal-log Digest, Vol 39, Issue 2


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>   1. I want to crystallize one protein with signal peptide on
>      N-terminal, Is it possible? (Mitra Kheirabadi)
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> ----------------------------------------------------------------------
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> Message: 1
> Date: Wed, 12 Nov 2008 11:30:17 -0800 (PST)
> From: Mitra Kheirabadi <mitrakheirabadi from yahoo.com>
> Subject: [Protein-crystallography] I want to crystallize one protein
> with signal peptide on N-terminal, Is it possible?
> To: xtal-log from magpie.bio.indiana.edu
> Message-ID: <140112.69149.qm from web36301.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi everybody
>
> I wanna crystallize a 16 kDa protein with signal peptide on the N-terminl, 
> is it correct? Protein activity is good with signal peptide but I don't 
> know if this signal peptide effect on 3D structure? Would you please guide 
> me?
>
> Best
>
> Mitra
>
>
>
>
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