Pichia Pastoris

Jaime Arenas jaime at seqvax.caltech.edu
Wed Aug 4 20:16:00 EST 1993


Hi all,

	I like to overexpress some S. cerevisiae proteins that are insoluble
in E. coli.  S. cerevisiae is an obvious alternative but usually the level
of expression is very low. I have heard of Pichia pastoris as an alternative 
with potential for very high leves of expression. I would appreciate some
advice and state of the art information on the P. pastoris expression system.

Is there a P. pastoris plasmid or expression constructs are simply integrated 
in the genome?

Thank for your help

Jaime E. Arenas
Division of Biology
CALTECH
Pasadena, Califonia

Jaime at seqvax.caltech.edu



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