yeast electroporation

NESTELBACHER Reinhard reinhard at wst.edvz.sbg.ac.at
Thu Dec 9 14:45:47 EST 1993


In article <CHqvJv.CHE at news.otago.ac.nz>, biocmag at sanger.otago.ac.nz (Michael Gieseg) writes:
Newsgroups: bionet.molbio.yeast
Mike Gieseg writes
	Does anybody out there have protocols for the electroporation of
Saccharomyces cerevisiae and Candida albicans.  I know they exist and if some 
kind person could Email them to me that would be great. Could someone also 
please tell me the addresses of archive sites containing methods and protocols
for Mol-biol techniques.

Thanks Heaps
Mike Gieseg
University of Otago
New Zealand
Email: biocmag at sanger.otago.ac.nz
__________________________________________________________

Dear Mike!

Do you have an electroporator??

1. An overview
   SHIGEKAWA-K,DOWER-W: BioTechniques Vol.6(8), pp. 742-750 (1988)

2. MEILHOC et al.: Bio/Technology 8, pp. 223-227 (1990)

3. LOROW-MURRAY-D: Focus 13, pp. 65-67  (1991)

4. BECKER-D, GUARENTE-L: Met. in Enz, Vol.194, pp. 182-187 (1991) very good!

5. Current Protocols in Molecular Biology

6. If you have an electroporator, look at the manual.Ours has a protocol for yeast ep.


If you find a newer and bether protocol, please inform us !!

Good luck!  Reinhard

NESTELBACHER Reinhard
reinhard at dgen10,gen.sbg.ac.at
University of Salzburg/Austria
Department of Genetics



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