electroporation

Manuel G. CLAROS claros at biologie.ens.fr
Thu Dec 9 06:53:57 EST 1993


In Article <01H69QUL5AU895MRR8 at Pap.UniVie.AC.AT>, Fatima Cvrckova
<FATIMA at AIMP.UNA.AC.AT> wrote:
>Dear Mike and others,
>
>a good yeast electroporation protocol has been published in Methods in
Enzymology,
>vol. 194, pp. 182-187, 1991.
>
>Fatima

What is not described in this protocol is that once you have prepared the cells,
the efficacity is improved 3 days later, and thet you can store 'electroporable'
cells in sorbitol 1M for 1 month without a big lost of efficacity, and usually
up tp 3 months.

Not at all

Manuel

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!  Manuel G. CLAROS                 !
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