max. size of integration plasmid?

Darin O'Brien obrien at
Wed Jul 7 17:11:55 EST 1993

Dear netters,

	I'm interested in integrating a test construct which is approximately 
22kb.  Can anyone tell me if this can be done with the standard integrating 
plasmids?  Will the typical integrating plasmid hold a 22kb insert?  Will it 
integrate it?  Will the integration be stably maintained?  As I am new to the 
field of yeast genetics/molecular biology, any and all comments would be 
greatly appreciated.  Perhaps YACs would be a better alternative?

Thanks much,

Darin (obrien at

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