max. size of integration plasmid?
obrien at molbio.cbs.umn.edu
Wed Jul 7 17:11:55 EST 1993
I'm interested in integrating a test construct which is approximately
22kb. Can anyone tell me if this can be done with the standard integrating
plasmids? Will the typical integrating plasmid hold a 22kb insert? Will it
integrate it? Will the integration be stably maintained? As I am new to the
field of yeast genetics/molecular biology, any and all comments would be
greatly appreciated. Perhaps YACs would be a better alternative?
Darin (obrien at molbio.cbs.umn.edu)
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