Integration plasmid (again)

Darin O'Brien obrien at molbio.cbs.umn.edu
Thu Jul 8 09:39:14 EST 1993


Thanks for the info on integration.  One response noted the following:

"To avoid integration at the selective marker, you could perhaps use a
strain bearing a deletion for the marker (for exemple his3deletion200, coming
from Sikorski and Hieter, Genetics, 1989, 122, 19-27)."

Is there a reason I should avoid integration of the test construct in the 
selective marker?  That's what I had originally planned to do.  More help 
please?!

Thanks much,

darin



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