Integration plasmid (again)

Darin O'Brien obrien at
Thu Jul 8 09:39:14 EST 1993

Thanks for the info on integration.  One response noted the following:

"To avoid integration at the selective marker, you could perhaps use a
strain bearing a deletion for the marker (for exemple his3deletion200, coming
from Sikorski and Hieter, Genetics, 1989, 122, 19-27)."

Is there a reason I should avoid integration of the test construct in the 
selective marker?  That's what I had originally planned to do.  More help 

Thanks much,


More information about the Yeast mailing list