LiAc transformation from SC media instead of YPD

Sandy Silverman sandy at nmr1.pt.cyanamid.COM
Thu Jul 15 08:06:58 EST 1993


To complicate matters further, cell density vs. OD is not linear over ~0.5
(again, depends on spec.).  For yeast transformation, I always use ~0.4
which is log.  Up to 0.8 (dilute to measure as per above) works but beyond
that I have found decreased efficiency.
--
Sanford Silverman                      >Opinions expressed here are my own<
American Cyanamid  
sandy at pt.cyanamid.com, silvermans at pt.cyanamid.com     "Yeast is Best"



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