a petition against ODs
dan at cubmol.bio.columbia.edu
Fri Jul 16 09:51:50 EST 1993
In article <QgFTR1K00iUy87xnEO at andrew.cmu.edu> "Morris F. Manolson" <mm6y+ at andrew.cmu.edu> writes:
>>In article <QgFJxqe00iV381eFZj at andrew.cmu.edu> mm6y+ at andrew.cmu.edu
>> Not every experiement needs an accurate cell count.
>Dear Dan, I agree completely! Thats why you only want to make
>one single graph of "OD vs Cell titer" for the entire time you
>use one particular spectrophotometer.
Except that OD/cell varies for each strain. We proably use 10-15 different
strains for our experiments (except mutant screen). You really need a
curve for each strain.
I take the point, but I wouldn't bother. I use OD, and I will publish
OD. I don't really care about the cell count.
My pet peeve: Journals that require centrifugation in g's. I don't know
g's from a whole in the head. I can report the speed, and the rotor. If
someone is cramping bad about it, let them go look up the radius. :-)
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