Gal1 mutants/AntimycinA

Kevin Morano ez005528 at ucdavis.edu
Wed Jul 28 14:22:58 EST 1993


Christoph Schller (CS at abc.univie.ac.at) wrote:
: Hi MS;RP;KM
: I used *10ppm Antimycin A* to sort out the ADH1-3 triplemutant in a
: cross. Worked nice.
: Cheers
: Christoph Schueller

For a purely practical explanation, we spread 100 ul of a 1 mg/ml stock
onto each plate, allow to dry and streak over that. Works for us to force
fermentative metabolism of sucrose as sole C source.


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Internet:kamorano at ucdavis.edu     "Why does it happen, because it happens..."
Bitnet:kamorano at ucdavis                                              N. Peart
Section of Microbiology, University of California, Davis



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