Stillman at BIOSCIENCE.BIOLOGY.UTAH.EDU
Fri Jul 30 17:13:37 EST 1993
Subject: Time: 2:42 PM
OFFICE MEMO genomic southerns Date: 7/29/93
Hi Yeast netters!
We have been having problems with restiction digests for southerns, and are
hoping somene will be able to help us.
We are trying to do a gene replacement in a specific strain, and to confirm the
gene replacement by southern blotting. The problem is that our DNA
preparations (made by the method of Hoffman and Winston Gene 57:267-272 (1987))
will not cut. This appears to be a strain specific effect, since DNA preps
from other strain backgounds cut fine.
The DNA preps contain a white precipitate which may be polysaccharides or
carbohydrates. Removing this precipitate by centrifugation seems to help the
digestion problem somewhat.
Have others encountered a similar problem? What solutions have you found for
Department of Cellular, Viral and Molecular Biology
University of Utah Medical Center
Salt Lake City, UT 84132
stillman at bioscience.utah.edu
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