EM of yeast

Morris F. Manolson mm6y+ at andrew.cmu.edu
Sat Jun 19 12:40:09 EST 1993


>    Can anybody suggest the growth conditions in which wild type
>strain cerevisiae enriches Golgi and ER membranes, so that it can be
>easier to identify them by immuno-gold electron microscopy.

For Golgi, you might intruduce a sec7 or sec14 mutation and grow
them for one hour at the non-permissive temperature before fixing.  Another
possibilty is to tranform with a high copy plasmid containing
the KEX2 gene.  Hope this helps, Morrie
******************************************************************************
Morris F. Manolson                     Tel:  416-813-6662  (office)
Division of Cell Biology                     416-813-5594  (lab)
Hospital for Sick Children                   416-813-5028  (FAX)
88 Elm St., McMaster building        email:  Morrie at resunix.ri.sickkids.on.ca
Toronto, Ontario, Canada
M5G 1X8



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