Mon Nov 1 18:43:15 EST 1993
Molecular Genetic Analysis of Genes
Required for Cytokinesis in
A post-doctoral position is available in January 1993 for an
initial period of two years. We have cloned genes required for
cytokinesis in Schizosaccharomyces pombe by complementation of
temperature-sensitive lethal cell division control mutants isolated
by Nurse, Thuriaux & Nasmyth (1976 Mol.Gen.Genet. 146:167-178).
One gene (cdc8) encodes a novel tropomyosin that forms an essential
part of the contractile ring at cytokinesis (Balasubramanian,
Helfman & Hemmingsen (1992) Nature 360: 84-87.
Ongoing research includes:
(1) A molecular genetic - biochemical study of tropomyosin
structures required for contractile ring functions.
We are carrying out a detailed mutagenesis of the cdc8
gene. We have found that some but not all fibroblast
tropomyosin isoforms derived from the rat _-tropomyosin
gene are capable of providing essential cdc8 tropomyosin
functions during cytokinesis. We are carrying out domain
swapping experiments based on these results.
(2) Identification of interacting genes and gene products using
various genetic approaches.
My lab is located in the Plant Biotechnology Institute of the
National Research Council Canada in Saskatoon. The position is
funded by a grant from the Medical Research Council. Other work in
the lab includes study of plant chloroplast chaperonin proteins.
I am looking for someone with interests in cell cycle control or
the cytoskeleton and expertise in yeast molecular genetics or
Sean Hemmingsen SHEMMING at PBI.NRC.CA
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