lichten%bchem.dnet at DXI.NIH.GOV
Mon Nov 1 07:54:51 EST 1993
Jens Alfken asks about zymolyase and the "yeast lytic enzyme" sold by ICN.
The first thing to get is the ICN 1992-1993 catalog. In it you will find a
more complete listing. ICN will be happy to sell you Zymolyase (either 20T
or 100T) which is repackaged from the original supplier. They also sell a
variety that they just call yeast lytic enzyme. All are prepared from
Arthrobacter luteus and all are (presumably) the same enzyme, just with
different levels of activity and different levels of contaminating
activities (i.e. proteases, DNases, RNases, etc.).
We find that, for routine DNA preparations, the yeast lytic enzyme (cat
#152270) is quite satisfactory and is by far the cheapest ($0.3 per 1K
units). We use it routinely. For more demanding purposes, such as
chromatin preparation, we still use zymolyase 100T (cat #32-093-1) which is
much more expensive ($12 per 1K units), and there are those in the
chromatin business who claim that you really should purify your own
lyticase to get rid of all the proteases. We have no experience with
spheroplast transformation, so I can offer you no help on that account.
You can see, however, that it's well worth your while to try both options.
If the cheaper enzyme will do, why not use it?
Michael Lichten, lichten at helix.nih.gov
Who started the business of these silly quotes at the end of a message?
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