zymolyase and alternatives
FATIMA at AT.AC.UNA.AIMP
Tue Nov 2 05:49:00 EST 1993
Dear Jens, Michael and others,
we are succesfully using snail digestive juice instead of zymolyase for
spheroplasting for transformation. It is being sold as "Glusulase" by NEN-Du Pont
(catalog: NEE154) for something like 110 $/10 ml vial (it is hard to compare units
but it means that you need about $ 0.11 worth per transformation). If you want to
save even more, you might collect your own snails and prepare the enzyme mix
yourself (yuk - I would not recommend it).
You probably need to modify the method (I don't know the zymolyase version) - if you
are interested, I can post our protocol.
Good luck! Fatima
More information about the Yeast