Yeast disruption

werner werner at JONAS.SACLAY.CEA.FR
Fri Nov 5 04:04:31 EST 1993


Dear Patrick,

I have in mind two such examples. One concerns CDC25 and the other the
53 kDa subunit of RNA polymerase III which is encoded by RPC53.
You can find the description of the problems the disruption caused
described in Petitjean et al. (1990) Genetics 124, 797-806) for
CDC25 and in Mann et al. (1992) Mol. Cell. Biol. 12, 4314-4326 and
in Chiannilkulchai et al. (1992) J. Biol. Chem. 267, 23099-23107.
In both cases the HIS3 insertion provided a promoter which allowed the
expression of the C-terminal part of the protein. In both cases
the C-terminal portion of the protein was sufficient to allow
the growth of the disrupted strain even though both genes are essential
as deletions showed.

Salut.

Michel Werner.

werner at jonas.sacaly.cea.fr





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