clumpy yeast

Tyrone Slothrop slothrop at macc.wisc.edu
Fri Nov 19 02:35:17 EST 1993


One of the yeast strain I am using displays a strange growth after I 
transform the cells with a plasmid carrying TRP marker and grow them in 
SD-TRP. They form clumps(or nice round balls) at the bottom of the tube.
Is this normal for some strains? Will this affect transformation
efficiency?
How should I deal with this?

Thanks for your attention.

Beilin Zhang (bzhang1 at students.wisc.edu)
Dept. of Biochemistry
UW-Madison



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