Chelex

Michael Lichten lichten%bchem.dnet at DXI.NIH.GOV
Wed Oct 6 14:11:40 EST 1993


From:	DXI::"BIOSCI-REQUEST at net.bio.net"  6-OCT-1993 07:35:20.48
To:	yeast at net.bio.net
CC:	
Subj:	Re: screening yeast by PCR

In regards to the question on screening yeast colonies by PCR we have used
a procedure for screening bacterial colonies and animal blood that should
work with just about anything.

1) Resuspend a small amount of cells from the colony (tip of a toothpick)
or 1-2 ul of whole animal blood in 100 ul sterile water.
2) place in boiling water bath for 10 minutes
3) spin 5' in the cold
4) use 2 in a 10ul or 5 ul in a 40ul  PCR reaction

If you have problems with signal BioRad sells a purified chelex that you
can add some to the boil that pulls out some inhibitors


Doug Rhoads                  || Dept. of Biological Sciences
drhoads at mercury.uark.edu     || 601 Science Engineering
drhoads at uafsysb.uark.edu     || University of Arkansas
501-575-3251                 || Fayetteville, AR 72701



More information about the Yeast mailing list