MET3 promoter

[Jim Sloan]

In article <2a6nef$lkl at agate.berkeley.edu>,
Randolph Hampton  <randy at mendel.berkeley.edu> wrote:
>We would like any information, anecdotes, advice about using the
>methionine-suppressable promoter in yeast.
>R. Hampton
>UC Berkeley MCB Dept
>510-642-7070

I found high levels of MET3 mRNA in yeast grown in glucose, but was 
unable to detect any MET3 mRNA in yeast grown on a non-fermentable carbon 
source (ie ethanol and glycerol).  Therefore it would appear that the 
MET3 promoter is regulated by carbon source.

Jim Sloan
jsloan at u.washington.edu