Need EcoRI/BamHI YEP

bwr at gnv.ifas.ufl.edu bwr at gnv.ifas.ufl.edu
Fri Sep 10 15:47:08 EST 1993


Dear Yeast Group,
	I'm a grad student looking for a particular type of plasmid so I can
express my maize gene in yeast. If anyone can help me out, please send me a fax
(904-392-6532), or E-Mail ("BWR at gnv.ifas.ufl.edu") or post a note here. Any
help greatly appreciated--here's what I'm looking for:
	* episomal plasmid, inducible expression.
	* cloning sites 5' EcoRI and 3' BamHI (finding this particular combo
		has been the hard part).
	* origins of replication for both S. cerevisiae and E. coli
	* typical genes for selection.

	Thanks in advance,
	Plant Pathology
	Fifield Hall
	University of Florida
	Gainesville, FL 32611 
	Bruce W. Ritchings



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