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Tue Sep 21 17:30:51 EST 1993

From: Roger Reeves <rreeves at>

Subject: postdoctoral position
To: yac at
Mime-Version: 1.0

Post-doctoral position available with Roger Reeves in the Department of 
Physiology, Johns Hopkins University School of Medicine.

	A project has been initiated to construct a physical map of mouse 
Chr16 with 100kb + 5 kb resolution.  The project involves somatic cell 
genetics to improve existing reduced hybrids; construction of a YAC library 
using state-of-the-art vectors and strains; standard contig construction 
methods coupled with high resolution backcross mapping to build the YAC 
contig, and the application of YAC fragmentation techniques to produce the 
high resolution map.  
	Allied with this is a search for genes.  Sources include genes 
identified by collaborators working on HSA21, 22,16 and 3 and collaboration 
with several efforts to isolate genes expressed at critical stages of 
embryogenesis and fetal development that map to mouse Chr16.  We are 
developing new ways to use homologous recombination in yeast to identify 
cloned sequences as well as adapting existing methods for use with YACs.  A 
new project directed by Phil Hieter and involving several labs at Hopkins, the 
National Library of Medicine and Indiana University involves cross-
referencing of expressed sequences from different organisms (yeast/human to 
start) and mapping of those sequences in mouse and human to build a gene 
rich map and to identify candidates for known mutations based on position in 
mammals and detailed knowledge of function in yeast. This project will 
provide some expressed sequences of known function in yeast and known 
position in mouse as markers on the emerging physical map.
	The YAC contig and genes identified using these reagents will be 
used to study effects of gene dosage on development.  We developed the 
initial procedure to introduce YACs into cells by spheroplast fusion and are 
now using this approach with ES cells to create defined dosage imbalance in 
mice.  Projects in my lab aand with a network of collaborators are looking at 
parameters influencing behaviour, immunology, neuronal generation and 
survival, Alzheimer Disease, cardiac development and function to you name 
it.  In addition, this is an extremely powerful system for positional cloning of 
genes whose effects can only be assayed in a complex organism, such as the 
weaver gene on mouse Chr16 which affects cerebellar development.  
	Finally, Hopkins School of Medicine is a great place to do genetics.  
We have an extremely rich environment for geneticists from a thriving yeast 
group to a variety of investigators in mammalian and human clinical and 
experimental genetics.  Two major international genetic databases (GDB and 
OMIM) are located here.  There are numerous first-rate seminar programs 
and lots of geneticists working in a very collegial environment.  Multi-
departmental graduate programs in Human Genetics and in Biochemistry, 
Cellular and Molecular Biology provide opportunities for many interactions 
among faculty from diverse areas of the School and Hospital.
	If you have a yeast/YAC/genetics background and are interested, 
please contact Roger Reeves at 
		Physiology 202
		Johns Hopkins University Schl of Medicine
		725 N. Wolfe St.
		Baltimore, MD 21205

	Phone: (410)955-6621
	FAX:    (410)955-0461
	email:  rreeves at

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