Tue Sep 21 17:30:51 EST 1993
From: Roger Reeves <rreeves at welchlink.welch.jhu.edu>
Subject: postdoctoral position
To: yac at net.bio.net
Post-doctoral position available with Roger Reeves in the Department of
Physiology, Johns Hopkins University School of Medicine.
A project has been initiated to construct a physical map of mouse
Chr16 with 100kb + 5 kb resolution. The project involves somatic cell
genetics to improve existing reduced hybrids; construction of a YAC library
using state-of-the-art vectors and strains; standard contig construction
methods coupled with high resolution backcross mapping to build the YAC
contig, and the application of YAC fragmentation techniques to produce the
high resolution map.
Allied with this is a search for genes. Sources include genes
identified by collaborators working on HSA21, 22,16 and 3 and collaboration
with several efforts to isolate genes expressed at critical stages of
embryogenesis and fetal development that map to mouse Chr16. We are
developing new ways to use homologous recombination in yeast to identify
cloned sequences as well as adapting existing methods for use with YACs. A
new project directed by Phil Hieter and involving several labs at Hopkins, the
National Library of Medicine and Indiana University involves cross-
referencing of expressed sequences from different organisms (yeast/human to
start) and mapping of those sequences in mouse and human to build a gene
rich map and to identify candidates for known mutations based on position in
mammals and detailed knowledge of function in yeast. This project will
provide some expressed sequences of known function in yeast and known
position in mouse as markers on the emerging physical map.
The YAC contig and genes identified using these reagents will be
used to study effects of gene dosage on development. We developed the
initial procedure to introduce YACs into cells by spheroplast fusion and are
now using this approach with ES cells to create defined dosage imbalance in
mice. Projects in my lab aand with a network of collaborators are looking at
parameters influencing behaviour, immunology, neuronal generation and
survival, Alzheimer Disease, cardiac development and function to you name
it. In addition, this is an extremely powerful system for positional cloning of
genes whose effects can only be assayed in a complex organism, such as the
weaver gene on mouse Chr16 which affects cerebellar development.
Finally, Hopkins School of Medicine is a great place to do genetics.
We have an extremely rich environment for geneticists from a thriving yeast
group to a variety of investigators in mammalian and human clinical and
experimental genetics. Two major international genetic databases (GDB and
OMIM) are located here. There are numerous first-rate seminar programs
and lots of geneticists working in a very collegial environment. Multi-
departmental graduate programs in Human Genetics and in Biochemistry,
Cellular and Molecular Biology provide opportunities for many interactions
among faculty from diverse areas of the School and Hospital.
If you have a yeast/YAC/genetics background and are interested,
please contact Roger Reeves at
Johns Hopkins University Schl of Medicine
725 N. Wolfe St.
Baltimore, MD 21205
email: rreeves at welchlink.welch.jhu.edu
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