G-418 selection

Neil Bromberg 5-5785 bromberg at ct.med.ge.com
Thu Sep 23 12:52:11 EST 1993


I am trying to find out how to use G-418 selection with electroporation for
yeast transformation.  Are cells selected directly on G-418 plates or is it 
added after a period of growth.  How many hours after plating?  I have tried
3 hours and 18 hours without much success.  Any suggestions will be greatly
appreciated.  

Please reply to:                              Susan Bromberg
Susan Bromberg                                1833 N. HiMount Blvd
bromberg at ct.med.ge.com                        Milwaukee WI 53208                        



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