sporulation problem

Daniel Zabetakis dan at cubmol.bio.columbia.edu
Mon Apr 4 16:06:17 EST 1994

In article <2npkbp$aq9 at charm.magnus.acs.ohio-state.edu>,
Tien-Hsien Chang <tchang at magnus.acs.ohio-state.edu> wrote:
> Any idea?  Thanks in advance for 
>hints and information.

  If what you are saying is that you have used your strain and conditions
successfully in the past, but it is not working now, I suggest you look at
the age of your plate you are taking the cells from to start. I have found 
that cells taken from old plates don't sporulate very well, even though 
I grow them up overnight in YPD.


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