sporulation problem

Daniel Zabetakis dan at cubmol.bio.columbia.edu
Mon Apr 4 16:06:17 EST 1994


In article <2npkbp$aq9 at charm.magnus.acs.ohio-state.edu>,
Tien-Hsien Chang <tchang at magnus.acs.ohio-state.edu> wrote:
> Any idea?  Thanks in advance for 
>hints and information.

  If what you are saying is that you have used your strain and conditions
successfully in the past, but it is not working now, I suggest you look at
the age of your plate you are taking the cells from to start. I have found 
that cells taken from old plates don't sporulate very well, even though 
I grow them up overnight in YPD.

DanZ

-- 
This article is for entertainment purposes only. Any facts, opinions,
narratives or ideas contained herein are not necessarily true, and do
not necessarily represent the views of any particular person.  




More information about the Yeast mailing list