High-efficiency mating protocols

Michael Lichten lichten%bchem.dnet at DXI.NIH.GOV
Fri Apr 8 07:51:04 EST 1994


David Rose asked about high-efficiency mating protocols.

This has worked for us in the past:

Grow the two cultures to saturation; mix at equal densities (important if 
one grows poorly) the liquid cultures.

Spot the mixed culture on a YEPD plate, grow overnight.

Streak (or resuspend and plate) selecting for the diploid.



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