electro-competent yeast

Michael Lichten lichten%bchem.dnet at DXI.NIH.GOV
Mon Apr 11 15:47:33 EST 1994


jbhogen asks about improving transformation frequencies using 
electroporation.

We have found, in our strain background (SK1), that despite what is said, 
addition of carrier DNA does make a difference (optimally, about 12.5-25 
micrograms of sonicated heterologous DNA/transformation)--about 10-fold 
with circular plasmids.  We had to optimize, though--higher amounts 
inhibited.  You might want to try that.  

michael lichten
lichten at helix.nih.gov



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