his3del200

Preston Garrison garrisonp at THORIN.UTHSCSA.EDU
Tue Apr 12 02:34:48 EST 1994


Scott J. Diede wrote:

>In article <2obs4u$6o6 at masala.cc.uh.edu>, bchs1b at Elroy.UH.EDU (Michael
>Benedik) wrote:
>
>> 
>> Can someone tell me the nature of the his3del200 mutation. Is this a deletion
>> of his, how big etc.
>> 
>> thanks
>> 
>> ----------------------------------------------------------------------
>>  Michael Benedik                              INTERNET: Benedik at uh.edu
>>  Dept. of Biochemical & Biophysical Sciences  
>>  University of Houston                                BITNET: Benedik at uhou
>> -----------------------------------------------------------------------
>
>The his3del200 allele consists of a 1 kb deletion that deletes the entire
>coding region within the genomic HIS3 containing EcoRI/SalI fragment cloned
>in the EcoRI and SalI sites of YRp14.
>

>I got this information from Sikorski and Hieter, Genetics 122:19-27 (May
>1989)
>The original allele construction is described in Fasullo and Davis, MCB
>8:4370-4380,1988 or Struhl and Hill, MCB 7:104-110, 1987.
>
>Hope this helps!
>
>
>
>****************************************************************************
>Scott J. Diede                                          
>The University of Chicago                               
>Department of Molecular Genetics and Cell Biology       
>920 E 58th Street Box 56                                
>Chicago IL 60637                                        
>TEL:  (312) 702-2031
>FAX:  (312) 702-3172
>died at midway.uchicago.edu                                                   
>****************************************************************************


Note also that this deletion removes part of the promoter of PET56, which is
upstream of HIS3, and this has effects on growth if the cells are on non-
fermentable carbon sources. See Science 262: 1886-1889 1993.

Preston Garrison
Biochem. Dept.
UTHSCSA
San Antonio, Tx 78284-7760
USA
garrisonp at uthscsa.edu



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