Interaction trap cloning

T. S. Pillay tpillay at
Mon Apr 18 23:44:52 EST 1994

1.  In the two-hybrid interaction trap cloning system using LexA fusion
proteins, does the size of colonies after Leu-  indicate the strength of
the interaction?

2.  After plating on X-gal plates- how soon should one get a positive
reaction which would indicate significant interaction - 1,2, 3 days

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