one-step gene disruptions

Dave Eide deide at UA.D.UMN.EDU
Tue Apr 19 07:27:52 EST 1994


Buddies,

We would like to do some one-step gene disruptions using flanking regions 
smaller than the recommended minimum.  Rothstein (Meth. Enzymol. vol. 194) 
suggests using flanking regions of 100 bp or more on each side of the selectable
marker and the method we want to try gives us only about 50 bp on each side.  
Does anyone have information regarding homologous recombination of fragments of 
this size?  Any input will be greatly appreciated.

Dave Eide 




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