reporter genes for yeast

Dave Eide deide at UA.D.UMN.EDU
Wed Apr 20 18:48:50 EST 1994


In message <9404202340.AA20822 at sequencer.wustl.edu> Mark Johnston writes:
> Hi buddies,
> 
>         As part of our sequencing project here in St. Louis, we are
> planning a large-scale gene disruption project.  In addition to disrupting
> genes, we would like also to construct promoter fusions to a reporter gene
> for measuring levels and regulation of gene expression.  I am writing to
> ask for advice on reporters to use.  The reporter must have the following
> properties:
> 
>         1.  small size (< ~ 2kb), so it is PCRable;
> 
>         2.  easy to assay (preferably with permealized cells);
> 
>         3.  relatively sensitive assay, (so it can detect expression from
> weak promoters);
> 
>         4.  no sequence homology to the yeast genome;  
> 
>         5.  (optional) provide a visual screen for activity;
> 
>         6.  (optional) provide a selection for and/or against function (for
> selecting 
>                                  regulatory mutants).
> 
> If anyone knows of a good reporter that has these properties, I'd
> appreciate hearing about it.  You can answer me directly, and I'll
> summarize the responses for the group.  Thanks.
> 
> Sincerely,
> 
> 
>         Mark Johnston
>         Department of Genetics Box 8232
>         Washington University Medical School
>         4566 Scott Ave.
>         St. Louis, MO  63110
>         FAX:   314-362-2985
>         TEL:    314-362-2735
>         E-mail:  mj at sequencer.wustl.edu

Mark,

How about green fluorescent protein?  Does anyone know if vectors are being 
developed for using GFP as a yeast reporter?

Dave Eide  
>             
> 
> 




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