reporter genes for yeast
deide at UA.D.UMN.EDU
Wed Apr 20 18:48:50 EST 1994
In message <9404202340.AA20822 at sequencer.wustl.edu> Mark Johnston writes:
> Hi buddies,
> As part of our sequencing project here in St. Louis, we are
> planning a large-scale gene disruption project. In addition to disrupting
> genes, we would like also to construct promoter fusions to a reporter gene
> for measuring levels and regulation of gene expression. I am writing to
> ask for advice on reporters to use. The reporter must have the following
> 1. small size (< ~ 2kb), so it is PCRable;
> 2. easy to assay (preferably with permealized cells);
> 3. relatively sensitive assay, (so it can detect expression from
> weak promoters);
> 4. no sequence homology to the yeast genome;
> 5. (optional) provide a visual screen for activity;
> 6. (optional) provide a selection for and/or against function (for
> regulatory mutants).
> If anyone knows of a good reporter that has these properties, I'd
> appreciate hearing about it. You can answer me directly, and I'll
> summarize the responses for the group. Thanks.
> Mark Johnston
> Department of Genetics Box 8232
> Washington University Medical School
> 4566 Scott Ave.
> St. Louis, MO 63110
> FAX: 314-362-2985
> TEL: 314-362-2735
> E-mail: mj at sequencer.wustl.edu
How about green fluorescent protein? Does anyone know if vectors are being
developed for using GFP as a yeast reporter?
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