Interaction trap cloning

[Andrew Mendelsohn]

In article <2ovnk4$59n at network.ucsd.edu>,
T. S. Pillay  <tpillay at ucsd.edu> wrote:
>1.  In the two-hybrid interaction trap cloning system using LexA fusion
>proteins, does the size of colonies after Leu-  indicate the strength of
>the interaction?
>
No.
>2.  After plating on X-gal plates- how soon should one get a positive
>reaction which would indicate significant interaction - 1,2, 3 days
>.....etc?
1 day for the strongest interactors, several for weaker ones.
andy mendelsohn
(brent lab)