YEAST VECTORS??

Manuel G. CLAROS claros at biologie.ens.fr
Mon Aug 1 14:02:08 EST 1994


In Article <n1436870019.20295 at mail1.ciwemb.edu>, meluh at MAIL1.CIWEMB.EDU
("Pam Meluh") wrote:

>I'm interested in any VECTORS you may know of that are for expression and
>purification of proteins in yeast, particularly heterologous proteins.  My
>colleague wants to express an Arabidopsis protein in yeast, as he has had
>difficulty doing so in E. coli.  Ideally, he would like something with a
>polylinker that allows in-frame cloning to an EPITOPE TAG (e.g. FLAG, GST,
>His-6) that can be exploited for purification.  The tag can be at the N- or
>C-terminus.  If you know of anything, please let me know ASAP.  Much thanks
>ahead of time. 

I think that you will find what you need in:

Reisdorf et al (1993), Current Genetics 23, 181-183.

I hope this helps

Manuel G.

M. G. Claros ------------------------------- claros at biologie.ens.fr
Ecole Normale Superieure  Genetique Moleculaire                      |/    \|
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