breaking yeast

[Alfredo Lopez]

In article:
>From: Susan Bromberg_- usmbcfm3 at ibmmail.com
>Research Department - Yeast Physiology and Fermentation
>414-931-4266
>Subject: breaking yeast



> I am trying to break open an industrialaneuploid yeast strain for enzyme
> assays.  This strain seem to much prefer remaining intact.  I tried twice
> through a French Press at 15,000psi and got only ~50% breakage or less.  I was
> thinking of going to glass beads but am worried about inactivating the enzyme.
> I would appreciate any suggestions.  Thanks

> Susan Bromberg


Unless lysozyme interferes with your assay, you can problably make speroplasts 
before using the French press.  Even with a bead beater it will be hard to get 
a 100% breakage.

	See you on the dark side of the Moon.
				Alfredo


------------------------------
Alfredo Lopez-De-Leon         |
lopez at medusa.bioc.aecom.yu.edu|
A.E.C.O.M.                    |
Biochemistry Department       |
Bronx, NY                     |
------------------------------