Want procedure to "float" spheroplasts on a gradient

Jeff jm15 at cornell.edu
Sat Aug 20 14:52:11 EST 1994


Greetings:

	I'm hoping that someone may have hands-on experience or good suggestions
for what type of solution (sucrose, etc. ?), concentration, and
centrifugation conditions that would allow AB1380 spheroplasts to float
in an osmotic solution.

	Thanks in advance to any helpful suggestions you might have and you may
Email me at jm15 at cornell.edu. if you like.

Cheers,

Jeff



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