Help! My plates won't gel

Charles Brenner brenner at rose.brandeis.edu
Wed Aug 24 09:02:04 EST 1994


genecutl at mendel.berkeley.edu (gc) writes:


>I've just started working with yeast again after a long
>hiatus and the first thing to do was, of course, make
>plates.  My YEPD plates were fine, but my minimal
>plates won't solidify.  They just become very soft and
>mushy after cooling down.  I first tried 20g agarose in
>1 liter of media and then the second time, I tried 25g
>with the same result.  I add the agarose, nitrogen base,
>amino acid dropout mix, and dextrose to a liter of water,
>autoclave for 30min and pour.
>Does anybody know what's going on here.  It's a bit hard
>to do yeast genetics if you can't make selective plates.

>Thanks.

>--gc

>...while making feet for children's shoes

Use AGAR instead of agarose.  Bacto-agar from Difco is the usual stuff.
CB
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