Help! My plates won't gel
brenner at rose.brandeis.edu
Wed Aug 24 09:02:04 EST 1994
genecutl at mendel.berkeley.edu (gc) writes:
>I've just started working with yeast again after a long
>hiatus and the first thing to do was, of course, make
>plates. My YEPD plates were fine, but my minimal
>plates won't solidify. They just become very soft and
>mushy after cooling down. I first tried 20g agarose in
>1 liter of media and then the second time, I tried 25g
>with the same result. I add the agarose, nitrogen base,
>amino acid dropout mix, and dextrose to a liter of water,
>autoclave for 30min and pour.
>Does anybody know what's going on here. It's a bit hard
>to do yeast genetics if you can't make selective plates.
>...while making feet for children's shoes
Use AGAR instead of agarose. Bacto-agar from Difco is the usual stuff.
Rosenstiel Center, rm 610 phone (617) 736-4944
Brandeis University fax (617) 736-2405
Waltham, MA 02254-9110 brenner at auriga.rose.brandeis.edu
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