immunofluor.-photobleaching

Ms. Cynthia Bolognese; BIOL; GRAD bolognes at umbc.edu
Thu Aug 25 11:24:52 EST 1994


First, I'd like to thank everyone for the advice on getting my yeast cells to 
stick to the slide. I've had some success. The problem now is bleaching. My
cells stain, I can see them in the periphery of the field of view, but as 
soon as I bring them into the center for a good view, they VERY RAPIDLY 
bleach. My secondary ab is DTAF conjugated (same excitation & emission 
wavelengths as FITC). Is this bleaching a result of unstable secondary? my 
staining technique? or the UV source? Any references or info would be greatly 
appreciated.  

Thanks in advance!

--
___________________________________________________________________________
Cindy Bolognese		 |						  |
e-mail address:  	 |    Qapla'!  (Klingon for Success!)             |
bolognes at umbc7.umbc.edu	 |						  |
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