Protease deficient strain for 2-Hybrid

Leon Parker mrclean at U.WASHINGTON.EDU
Sun Aug 28 14:36:04 EST 1994


Hello all--

We suspect that the fusion proteins we have created for use in the yeast 
2-hybrid system are being degraded when expressed in the strains 
used for screening libraries.    Is anyone familiar with a protease 
deficient yeast strain that would be compatible with either Stan Fields' 
2-hybrid assay or Roger Brent's interaction trap protocol?  Strain 
designations and/or contact names would be greatly appreciated.  

Please respond directly to: mrclean at u.washington.edu

Thanks in advance.

--Leon Parker
Department of Microbiology
University of Washington
Mail Stop SC-42
Seattle, WA  98195
(206) 543-8440



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