trp1 and his3 transplacement plasmids

ZEYL,CLIFFORD WESLEY,MR B7JM000 at MUSICB.MCGILL.CA
Wed Aug 31 19:30:38 EST 1994


Dear buddies,
   Sikorski and Hieter (Genetics 122: 19-27, 1989) describe
the construction of a bunch of vectors and budding yeast strains
for transformation.  To knock out the wild-type genes in their
starting strains they made some transplacement vectors carrying
URA3 and deleted copies of trp1 and his3 (plasmids YRp14/trp1-
delta1 and YRp14/his3-delta200, respectively, in the paper).
Does anyone out there have these plasmids, or know where I
can get them?
               Cliff Zeyl
               b7jm at musicb.mcgill.ca




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