changing mating type

Joanne Tornow jtornow at whale.st.usm.edu
Fri Dec 9 17:56:06 EST 1994


I have received several responses to my query about changing
mating type. I want to thank everyone for their help. For
those who didn't know, this can be accomplished by
transforming with a plasmid containing the HO gene (either
wildtype or under control of the GAL promoter), allowing
switching, and then curing the cells of the plasmid.
A detailed discussion can be found in Herskowitz and Jenson,
Meth. Enzymol. 194:132. Thanks again to everyone who
responded.

Joanne Tornow



More information about the Yeast mailing list