Membrane proteins in dihybrid?

Thomas David Fox tdf1 at CORNELL.EDU
Fri Dec 9 10:08:42 EST 1994

We had good luck using the two-hybrid system to detect interactions among
proteins associated with the inner mitochondrial membrane.  One of the
proteins, PET122, doesn't have an obvious membrane spanning domain but
behaves like an integral membrane protein (when overproduced).  Another,
PET494, also appears to be tightly associated with the membrane, but PET494
only worked when a putative membrane spanning domain was deleted.  The
third, PET54, is soluble and peripherally associated with the membrane.

Tom Fox

Brown, N. G., M. C. Costanzo and T. D. Fox, 1994  Interactions among three
proteins that specifically activate translation of the mitochondrial COX3
mRNA in Saccharomyces cerevisiae. Mol. Cell. Biol. 14: 1045-1053.

McMullin, T. W. and T. D. Fox, 1993  COX3 mRNA-specific translational
activator proteins are associated with the inner mitochondrial membrane in
Saccharomyces cerevisiae. J. Biol. Chem. 268: 11737-11741.

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