RNA preps

dave eide deide at D.UMN.EDU
Wed Dec 14 13:49:54 EST 1994


Hi all,

We are studying a gene expressed at low levels.  I'm interested in preparing 
large amounts of total S. cerevisiae RNA from cells grown under 4 or 5
different conditions with the purpose of making polyA+ RNA for 
Northerns.  Can anyone recommend an RNA prep that yields about 10 mgs of 
total RNA to use for polyA+ selection?

Thanks for any help

Dave Eide



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