Glucose reppression of gal promoters

Colin Rasmussen ras-1 at bones.biochem.ualberta.ca
Sat Dec 17 00:04:17 EST 1994


> A friend has an essential gene behind a gal promoter.  Shift to glucose
> does not kill the cells.  Is this likely suppressor mutations or
> readthrough?  No RNA can be detected on glucose.
> Respond here or by email to him directly at aitchij at rockvax.rockefeller.edu
> Thank you.
> 
After how long in glucose ?  If it is an especially stable protein, there 
might be sufficient amoounts around for quite a while.  Another 
possibility is that it is required for the transition from quiescence but 
not for vegetative growth.

Colin



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