Glucose reppression of gal promoters
ras-1 at bones.biochem.ualberta.ca
Sat Dec 17 00:04:17 EST 1994
> A friend has an essential gene behind a gal promoter. Shift to glucose
> does not kill the cells. Is this likely suppressor mutations or
> readthrough? No RNA can be detected on glucose.
> Respond here or by email to him directly at aitchij at rockvax.rockefeller.edu
> Thank you.
After how long in glucose ? If it is an especially stable protein, there
might be sufficient amoounts around for quite a while. Another
possibility is that it is required for the transition from quiescence but
not for vegetative growth.
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