Glucose reppression of gal promoters

Richard Buckholz RGB12955 at USAV01.GLAXO.COM
Mon Dec 19 14:21:27 EST 1994

Is the gal promoter-essential gene construct on a 2u plasmid?  If so and if
the gene pproduct is only needed in small quantities, the less stringent 
control of gal promoters on high-copy vectors might be causing the problem,
especially if your friend is doing the lethal switch on plates instead of 

Richard G. Buckholz
Glaxo Research Institute
Five Moore Drive
Research Triangle Park, NC  27709
Internet:  RGB12955 at

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