CHAZ andre at mpimg-berlin-dahlem.mpg.de
Wed Dec 21 13:43:24 EST 1994

Does anybody have suggestions for preparing mitochondria
from S. cereviseae?  I have been making spheroplasts with
zymolase, but I have trouble breaking the cells open-
I tried a tissue homogenizer, but even after 30 strokes
the yeast seem to be quite happy and intact.  Any other
ideas?   Thanks in advance for suggestions.

C. Andre

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