Casamino acids to EMM medium?
moser at U.WASHINGTON.EDU
Wed Dec 21 12:48:11 EST 1994
On 20 Dec 1994, Michael Benedik wrote:
> I was just wondering if adding casamino acids to EMM minimal medium for
> growing S. pombe will allow faster growth. Since we are mostly doing
> Ura selections this should work fine in theory, just want to know what
> pombe things of casamino acids.
I study pombe in a lab where every one else studies cerevisiae. I tried
growing pombe on our version of SD medium which basically consists of
Difco yeast Nitrogen Base (YNB) + Casamino Acids (+Trp, Ura or Ade if
needed). It grew much slower than on EMM. I then found that fission
yeast actually will grow well on YNB with only the supplements needed (in
my case Ade, Ura, His and Leu). I call this media NBA or Nitrogen Base
Agar after the following reference (Ponticelli, A. S. and Smith, G. R.
(1989) Genetics 123, 45-54) except I use 2% glucose. I hope this helps.
Mike Moser Tel: 206-543-5354
Department of Biochemistry SJ-70 FAX: 206-685-1792
University of Washington moser at u.washington.edu
Seattle, WA 98195 Make peace my beast is yeast
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