Salmon Sperm DNA for transformation

Seth Findley findley at u.washington.edu
Wed Feb 9 14:32:24 EST 1994


Is there an optimal size for sonicated salmon sperm DNA for 
transformation via Lithium/PEG ?

Current Protocols mentions that a range from 2-12 kb with a peak around 
7kb for the smear (on a minigel) is good, but then in the same paragraph 
it says bigger is better.

Is this just a trade of efficiency versus viscosity (handling?)

If a less-sonicated prep is diluted to 1mg/ml (from 10mg/ml) in fact better?

Thanks.

-Seth

HHMI
UofW
Seattle



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