GAL/Nde vectors

Jaime Arenas jaime at seqvax.caltech.edu
Mon Feb 14 13:41:00 EST 1994


Dear buddies,

	I need to subclone a gene under the gal promoter. The ovbious thing
to do is to completely delete your own promoter and then clone it as close
as possible to the gal1/10 fragment.  With this it also become obvious that
a GAL1/10 construct that have NdeI in one side and NcoI in the other side
of the Gal1/10 fragment would be a great thing to have. Is there anyone
out there that already have such construct or something alike? If so, could
I have an aliquot?

Thanks for your promt response,

Jaime Arenas
Division of Biology 147-75
CALTECH




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